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khchao.com
Kuan-Hao Chao

Side project

CRISPR Commander

A molecular precision arcade: match the PAM, switch your nuclease, and protect a bacterial cell. Learn the targeting rules in three short practice rounds, then take on endless mixed DNA.

Browser arcade · keyboard & touch

Molecular precision arcade

Cas9 · guide → NGG   /   Cas12a · TTTV → guide

Score0
Best0
ATP Pool60 ATP
Combo Multiplier1x NORMAL
Cell integrity: 100%
Active Nuclease:

Practice 1/3 · Cas9: guide → NGG

How to Play & Molecular Mechanics

  • Slice to cleave: Drag or swipe across a paired DNA ribbon. Lift between hits on armored targets. Cas9 is shown with a qualitative blunt break; Cas12a with a staggered break.
  • PAM Specificity: Match the active Cas enzyme to the target's PAM motif. SpCas9 cleaves targets with NGG; AsCas12a cleaves T-rich TTTV motifs.
  • Enzyme Hotkeys: Press 1 or 2 on desktop (or tap the enzyme buttons) to switch Cas complexes in real time.
  • Guides are preloaded: A complementary guide RNA is assumed for every target. A PAM is necessary, but by itself is not sufficient for real CRISPR targeting. Displayed DNA is a schematic, not a structural simulation.
  • Practice: Complete three cuts each with Cas9, Cas12a, then mixed targets. Practice breaches do not cause damage. Score and powers reset before ranked endless play.
  • Combos: Every five uninterrupted cuts adds 0.5× to the multiplier, up to 4×. Breaches cost 10% integrity, or 30% for a boss. AcrIIA4 inhibits Cas9; switch to the boss's Cas12a-compatible DNA target.
  • Fictional tactical powers: Shield slows targets, repair restores 40 integrity, target assist enlarges the cutting tolerance, and overdrive makes periodic compatible cuts. None is a claim about base editing, prime editing, or a universal cellular defense.