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Kuan-Hao Chao

Side project

CRISPR Commander

Take command of CRISPR-Cas molecular machinery. Slice invading viral DNA strands at specific Protospacer Adjacent Motifs (PAM) before they integrate into the host bacterial genome.

CRISPR-Cas Ribonucleoprotein Complex

Target and cleave viral DNA strands at PAM motifs: NGG (SpCas9) and TTTV (AsCas12a).

Score0
Best0
ATP Pool60 ATP
Combo Multiplier1x NORMAL
Host Genome Viability: 100%
Active Nuclease:

How to Play & Molecular Mechanics

  • Slice to Cleave: Drag your mouse or swipe on mobile across falling viral nucleic acid strands to execute a double-strand break (DSB).
  • PAM Specificity: Match the active Cas enzyme to the target's PAM motif. SpCas9 cleaves targets with NGG; AsCas12a cleaves T-rich TTTV motifs.
  • Enzyme Hotkeys: Press 1 or 2 on desktop (or tap the enzyme buttons) to switch Cas complexes in real time.
  • Combos & Multipliers: Chain multiple successful cleaves without letting strands hit the bottom integration line to build up your streak multiplier and earn bonus ATP.
  • Tactical Cellular Overdrives: Spend ATP on dCas9 Shield (blocks integration), Prime Edit (+40 health), Base Edit (converts C→T), or Hyper-Drive (high-speed clearing).